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    Allegrow Biotech Logo
    Allegrow Biotech Logo
    • Products 
      • AimGel
      • Cells
    • Technology 
      • Overview
      • Deep Dive
    • About 
      • Our Team
      • Our Story
      • News
      • Contact
    • Resources 
      • Product Resources
      • Investor Teaser
      • AimGel Builder
    • …  
      • Products 
        • AimGel
        • Cells
      • Technology 
        • Overview
        • Deep Dive
      • About 
        • Our Team
        • Our Story
        • News
        • Contact
      • Resources 
        • Product Resources
        • Investor Teaser
        • AimGel Builder
      Where to Buy
      Allegrow Biotech Logo
      • AimGel Dog T Cell Activator

        Dog T cell activation on cell-sized hydrogel microparticles

        AimGel Dog T Cell Activator

        AimGel Dog T Cell Activator

        $698.00
        ATC01-0050D | Research Use Only

        AimGel Dog T Cell Activator presents anti-CD3 and anti-CD28 signals on soft hydrogel microparticles built to the size of a real dog T cell (7-12 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell. Activation is gentle and effective, whether you start from isolated dog T cells or straight from PBMC.

        1 vial of AimGel Dog T Cell Activator, 0.5 mL in PBS at 4 × 10⁷ microparticles/mL
        Quantity
        Coming soon
      • AimGel biomimicry

        Canine T cells clustering around the red (rhodamine) labelled AimGel microparticles decorated with anti-CD3 and anti-CD28 signals.

      • Product Details

        Specifications

        A suspension of AimGel hydrogel microparticles made of chemically crosslinked dextran. Microparticles sized ~7-12 µm, coated with a phospholipid bilayer, with dog T cell activating signal panels docked to the membrane.

        Inside the kit

        • 1 vial of AimGel Dog T Cell Activator in PBS at 4 × 10⁷ microparticles/mL, with 0.5% P/S
        • 1 vial of lysis buffer (100×), dextranase in PBS

        Storage & stability

        • Ships on ice
        • Do not freeze, keep refrigerated at 2–8 °C, stable for 12 months.
        • Contents are sterile when unopened

        Documents

        • Protocol

        You will also need

        • Cryopreserved/fresh dog PBMC, or isolated dog CD3⁺ T cells
        • RPMI 1640 supplemented with 2 mM L-glutamine,
        • 10% fetal bovine serum (FBS)
        • 25 mM HEPES
        • 1% penicillin/streptomycin
        • 25 µM 2-mercaptoethanol
        • Recombinant human IL-2
        • Cell culture vessels
        • Humidified CO₂ incubator or bioreactor
      • Data

        Section image

        Expand straight from PBMC

        Expansion of dog PBMC with AimGel Dog T Cell Activator compared with well plates coated with dog aCD3/28 antibodies and negative control. AimGel with dog aCD3/28 antibodies successfully triggers the expansion of dog T cells. Controls (coated plate and negative) done with donor 1 only.

        Section image

        Dog cell viability

        Cells (PBMC) maintain high viability post-activation, with 2 dog donors. Compared with coated plate and no activation controls (donor 1 only).

      • FAQs

        Can I start from dog PBMC, or do I need isolated T cells?

        Either. AimGel selectively expands CD3+ T cells, so a separate isolation step is often unnecessary. See the protocol for seeding guidance for each starting material.

        How do I remove the AimGel microparticles?

        Add the included lysis buffer. Dextranase fully degrades the microparticles in 30 minutes at 37 °C, without affecting your cells. See the protocol for timings.
        If you need AimGel that clears on its own at a set point, we can formulate it to hydrolyse under your culture conditions, get in touch.

        Can I re-stimulate my dog T cells?

        Yes. Growth typically slows after day 10, and re-stimulating promotes continued expansion with minimal activation-induced exhaustion. We recommend an AimGel-to-cell ratio of 0.25:1 for re-stimulation.
        Do not use the lysis buffer if you plan to re-stimulate, as residual dextranase will degrade freshly added AimGel unless it is thoroughly washed out.

        Can I cryopreserve the expanded cells?

        Yes. Degrade the AimGel microparticles first, then wash the cell pellet at least twice before cryopreservation to remove residual dextranase.
      • Order Now

        Purchase through our distributors, or our webstore if no distributor is serving your region.

        LAB-A-PORTER logo, distributor for Hong Kong

        LAB-A-PORTER

        Hong Kong

        Buy on LAB-A-PORTER
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        Lab Panda

        Hong Kong

        Contact Lab Panda

        Allegrow Webstore

        Other regions

        Allegrow Webstore
      • Other Activators in the AimGel lineup

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        Human T Cell Activator

        T cell activation and expansion straight from PBMC. Drop-in replacement for CD3/CD28 beads with superior performance.

        Learn More
        Section image

        Human NK Cell Activator

        Feeder-free NK cell activation and expansion.

        Learn More
        Section image

        Mouse T Cell Activator

        Activate mouse T cells with AimGel artificial cells, straight from PBMC.

        Learn More
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        Aim-Core

        Blank artificial cells for custom applications. Dock your own biotinylated signals.

        Learn More
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