• AimGel Dog T Cell Activator

    Dog T cell activation on cell-sized hydrogel microparticles

    AimGel Dog T Cell Activator

    AimGel Dog T Cell Activator

    $698.00
    ATC01-0050D | Research Use Only

    AimGel Dog T Cell Activator presents anti-CD3 and anti-CD28 signals on soft hydrogel microparticles built to the size of a real dog T cell (7-12 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell. Activation is gentle and effective, whether you start from isolated dog T cells or straight from PBMC.

    1 vial of AimGel Dog T Cell Activator, 0.5 mL in PBS at 4 × 10⁷ microparticles/mL
    Quantity
    Coming soon

  • Performance

    Microparticle-based activation

    A convenient mix-and-use reagent for dog T cell activation and expansion. Gone are the days of coating well-plates with antibodies to obtain activated dog T cells.

    Built to mimic an antigen-presenting cell

    The soft hydrogel core matches the mechanical stiffness and size of a real cell, while a mobile lipid bilayer lets activation signals travel across the surface just as they would on a natural APC. That biomimicry is why AimGel needs significantly fewer activation signals than rigid magnetic beads while activating more effectively, and also more gently.

    Self-degradable via hydrolysis

    As hydrogel microparticles, AimGel self-degrades via hydrolysis within 7-10 days, so a standard expansion needs no debeading step. Need them gone sooner? The included digesting buffer clears them in 30 minutes at 37 °C.

  • Product Details

    Specifications

    A suspension of AimGel hydrogel microparticles made of chemically crosslinked dextran. Microparticles sized ~7-12 µm, coated with a phospholipid bilayer, with mouse T cell activating signal panels docked to the membrane.

    Inside the kit

    • 1 vial of AimGel Dog T Cell Activator in PBS at 4 × 10⁷ microparticles/mL, with 0.5% P/S
    • 1 vial of lysis buffer (100×), dextranase in PBS

    Storage & stability

    • Ships on ice
    • Do not freeze, keep refrigerated at 2–8 °C, stable for 12 months.
    • Contents are sterile when unopened

    You will also need

    • Cryopreserved/fresh dog PBMC, or isolated dog CD3⁺ T cells
    • RPMI 1640 supplemented with 2 mM L-glutamine,
    • 10% fetal bovine serum (FBS)
    • 25 mM HEPES
    • 1% penicillin/streptomycin
    • 25 µM 2-mercaptoethanol
    • Recombinant human IL-2
    • Cell culture vessels
    • Humidified CO₂ incubator or bioreactor

  • Data

    Section image

    Dog cell proliferation

    Expansion of dog PBMC with AimGel Dog T Cell Activator compared with well-plates coated with dog aCD3/28 antibodies. AimGel with dog aCD3/28 antibodies successfully triggers the expansion of dog T cells.

    Section image

    Dog cell viability

    Mouse T cells activated with AimGel, starting from mouse PBMC. Left: viability remained between 80% and 92% across days 3, 5 and 7. Centre: CD3+ fraction of live cells rose from ~14% at day 0 to ~98% by day 5, showing selective expansion of T cells without a separate isolation step. Right: ~98% of the CD3+ population was CD25+ at day 5, confirming activation.

  • AimGel FAQs

  • Order Now

    Purchase through our distributors, or our webstore if no distributor is serving your region.

    LAB-A-PORTER logo, distributor for Hong Kong

    LAB-A-PORTER

    Hong Kong

    Allegrow Webstore

    Other regions

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