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AimGel Dog T Cell Activator
Dog T cell activation on cell-sized hydrogel microparticles


AimGel Dog T Cell Activator
$698.00
ATC01-0050D | Research Use Only
AimGel Dog T Cell Activator presents anti-CD3 and anti-CD28 signals on soft hydrogel microparticles built to the size of a real dog T cell (7-12 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell. Activation is gentle and effective, whether you start from isolated dog T cells or straight from PBMC.
1 vial of AimGel Dog T Cell Activator, 0.5 mL in PBS at 4 × 10⁷ microparticles/mLQuantityComing soonAimGel biomimicry
Canine T cells clustering around the red (rhodamine) labelled AimGel microparticles decorated with anti-CD3 and anti-CD28 signals.
Product Details
Specifications
A suspension of AimGel hydrogel microparticles made of chemically crosslinked dextran. Microparticles sized ~7-12 µm, coated with a phospholipid bilayer, with dog T cell activating signal panels docked to the membrane.
Inside the kit
- 1 vial of AimGel Dog T Cell Activator in PBS at 4 × 10⁷ microparticles/mL, with 0.5% P/S
- 1 vial of lysis buffer (100×), dextranase in PBS
Storage & stability
- Ships on ice
- Do not freeze, keep refrigerated at 2–8 °C, stable for 12 months.
- Contents are sterile when unopened
Documents
You will also need
- Cryopreserved/fresh dog PBMC, or isolated dog CD3⁺ T cells
- RPMI 1640 supplemented with 2 mM L-glutamine,
- 10% fetal bovine serum (FBS)
- 25 mM HEPES
- 1% penicillin/streptomycin
- 25 µM 2-mercaptoethanol
- Recombinant human IL-2
- Cell culture vessels
- Humidified CO₂ incubator or bioreactor
Data

Expand straight from PBMC
Expansion of dog PBMC with AimGel Dog T Cell Activator compared with well plates coated with dog aCD3/28 antibodies and negative control. AimGel with dog aCD3/28 antibodies successfully triggers the expansion of dog T cells. Controls (coated plate and negative) done with donor 1 only.

Dog cell viability
Cells (PBMC) maintain high viability post-activation, with 2 dog donors. Compared with coated plate and no activation controls (donor 1 only).
FAQs
Can I start from dog PBMC, or do I need isolated T cells?
Either. AimGel selectively expands CD3+ T cells, so a separate isolation step is often unnecessary. See the protocol for seeding guidance for each starting material.
How do I remove the AimGel microparticles?
Add the included lysis buffer. Dextranase fully degrades the microparticles in 30 minutes at 37 °C, without affecting your cells. See the protocol for timings.
If you need AimGel that clears on its own at a set point, we can formulate it to hydrolyse under your culture conditions, get in touch.
Can I re-stimulate my dog T cells?
Yes. Growth typically slows after day 10, and re-stimulating promotes continued expansion with minimal activation-induced exhaustion. We recommend an AimGel-to-cell ratio of 0.25:1 for re-stimulation.
Do not use the lysis buffer if you plan to re-stimulate, as residual dextranase will degrade freshly added AimGel unless it is thoroughly washed out.
Can I cryopreserve the expanded cells?
Yes. Degrade the AimGel microparticles first, then wash the cell pellet at least twice before cryopreservation to remove residual dextranase.
Order Now
Purchase through our distributors, or our webstore if no distributor is serving your region.
LAB-A-PORTER
Hong Kong
Lab Panda
Hong Kong
Allegrow Webstore
Other regions
Other Activators in the AimGel lineup
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Mouse T Cell Activator
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Aim-Core
Blank artificial cells for custom applications. Dock your own biotinylated signals.









