• AimGel Mouse T Cell Activator

    Mouse T cell activation on cell-sized hydrogel microparticles

    AimGel Mouse T Cell Activator

    AimGel Mouse T Cell Activator

    $150.00
    ATC01-0050M | Research Use Only

    AimGel Mouse T Cell Activator presents anti-CD3 and anti-CD28 signals on soft hydrogel microparticles built to the size of a real mouse T cell (7-12 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell. Activation is gentle and effective, whether you start from isolated mouse T cells or straight from PBMC.

    1 vial, 0.5 mL in PBS at 4 × 10⁷ microparticles/mL
    Quantity
    Coming soon

  • AimGel biomimicry

    Mouse T cells clustering around AimGel microparticles decorated with anti-CD3 and anti-CD28 signals.

  • Our mouse T cell activator is built on our AimGel artificial cell technology, mimicking the immunological synapse for better performance: 47-fold expansion straight from PBMC, reaching ~98% CD3+ purity by day 5.

  • Product Details

    Specifications

    A suspension of AimGel hydrogel microparticles made of chemically crosslinked dextran. Microparticles sized ~7-12 µm, coated with a phospholipid bilayer, with mouse T cell activating signal panels docked to the membrane.

    Inside the kit

    • 1 vial of AimGel Mouse T Cell Activator in PBS at 4 × 10⁷ microparticles/mL, with 0.5% P/S
    • 1 vial of lysis buffer (100×), dextranase in PBS

    Storage & stability

    • Ships on ice
    • Do not freeze, keep refrigerated at 2–8 °C, stable for 12 months.
    • Contents are sterile when unopened

    Documents

    You will also need

    • Cryopreserved/fresh mouse PBMC, or isolated mouse CD3⁺ T cells
    • RPMI 1640 supplemented with 2 mM L-glutamine,
    • 10% fetal bovine serum (FBS)
    • 25 mM HEPES
    • 1% penicillin/streptomycin
    • 25 µM 2-mercaptoethanol
    • Recombinant mouse or human IL-2
    • Cell culture vessels
    • Humidified CO₂ incubator or bioreactor

  • Data

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    Start with mouse PBMC

    Expansion of mouse PBMC with AimGel Mouse T Cell Activator from PBMC, reaching 47-fold expansion by day 7.

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    Viability, purity and activation

    Mouse T cells activated with AimGel, starting from mouse PBMC. Left: cell viability remained between 80% and 92% across days 3, 5 and 7. Centre: CD3+ fraction of live cells rose from ~14% at day 0 to ~98% by day 5, showing selective expansion of T cells without a separate isolation step. Right: ~98% of the CD3+ population was CD25+ at day 5, confirming activation.

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    What about second stimulation?

    Thanks to gentle and biomimetic signal presentation, AimGel for mouse T cells is able to effectively restimulate the expansion of mouse T cells. Mouse PBMC were initially stimulated with AimGel for mouse T cells, and were then restimulated with AimGel for mouse T cells on day 11, except for the negative control.

  • FAQs

  • Order Now

    Purchase through our distributors, or our webstore if no distributor is serving your region.

    LAB-A-PORTER logo, distributor for Hong Kong

    LAB-A-PORTER

    Hong Kong

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    Lab Panda

    Hong Kong

    Allegrow Webstore

    Other regions

  • Other Activators in the AimGel lineup

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    Human T Cell Activator

    T cell activation and expansion straight from PBMC. Drop-in replacement for CD3/CD28 beads with superior performance.

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    Human NK Cell Activator

    Feeder-free NK cell activation and expansion.

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    Dog T Cell Activator

    Activate dog T cells with AimGel artificial cells, straight from PBMC.

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    Aim-Core

    Blank artificial cells for custom applications. Dock your own biotinylated signals.

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