• AimGel Human T Cell Activator

    CD3/CD28/CD137 activation on cell-sized hydrogel microparticles

    AimGel Human T Cell Activator

    AimGel Human T Cell Activator

    $150.00
    ATC01-0050H | Research Use Only

    AimGel Human T Cell Activator presents anti-CD3, anti-CD28 and anti-CD137 signals on soft hydrogel microparticles built to the size of a real cell (10-15 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell — so activation is gentle and effective, whether you start from isolated T cells or straight from PBMC.

    1 vial of AimGel Human T Cell Activator, 0.5 mL in PBS at 4 × 10⁷ microparticles/mL
    Quantity
    Coming soon

  • Performance

    Quantity and quality

    AimGel yields 60% more T cells than existing synthetic solutions such as magnetic beads and nanomatrix, in less time, with matching viability (n=4, PBMCs from healthy human donors). Because the signals are presented gently rather than forced, cells come through with less exhaustion, they stay responsive, and you can re-stimulate.

    Built to mimic an antigen-presenting cell

    The soft hydrogel core matches the mechanical stiffness and size of a real cell, while a mobile lipid bilayer lets activation signals travel across the surface just as they would on a natural APC. That biomimicry is why AimGel needs significantly fewer activation signals than rigid magnetic beads while activating more effectively, and also more gently.

    Self-degradable via hydrolysis

    As hydrogel microparticles, AimGel self-degrades via hydrolysis. So say goodbye to pesky debeading steps and hello to a streamlined workflow. Need them gone sooner? The included lysis buffer clears them on demand.

  • Product Details

    Specifications

    A suspension of AimGel hydrogel microparticles made of chemically crosslinked dextran. Microparticles sized ~10-15 µm, coated with a phospholipid bilayer, with human T cell activating signal panels docked to the membrane.

    Inside the kit

    • 1 vial of AimGel Human T Cell Activator in PBS at 4 × 10⁷ microparticles/mL, with 0.5% P/S
    • 1 vial of lysis buffer (100×), dextranase in PBS

    Storage & stability

    • Ships on ice
    • Do not freeze, keep refrigerated at 2–8 °C, stable for 12 months.
    • Contents are sterile when unopened

    You will also need

    • Cryopreserved or freshly isolated T cells, or CD3⁺ T cells
    • RPMI 1640 supplemented with 2 mM L-glutamine, or equivalent
    • Heat-inactivated fetal bovine serum (FBS)
    • Penicillin/streptomycin
    • Recombinant human IL-2 (rIL-2)
    • 2-Mercaptoethanol
    • Cell culture vessels
    • Humidified CO₂ incubator or bioreactor

  • Data

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    Proliferation and viability

    AimGel outperforms existing synthetic solutions such as magnetic beads and nanomatrix by 60%, yielding more T cells in a shorter time. Viability of T cells activated via AimGel matches current synthetic solutions. n=4, PBMCs from healthy human donors.

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    Memory subtypes

    T cell memory subtypes of PBMCs activated by AimGel and other popular T cell activation reagents. AimGel produces more even fractions of effector memory and central memory subtypes.

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    Activation markers

    Expression of early-stage marker (CD69) and late-stage marker (CD25), indicating T cell activation. Unstimulated cells used as gating.

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    CAR-T function

    In vitro CAR-T cell mediated killing assessed by fluorescent microscopy of GFP⁺ Raji cancer cell lines co-cultured with CAR-T cells.

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    Degradation timecourse

    Bright-field micrographs showing activation and expansion of pan T cells at days 2, 3, 5 and 7. Scale bar = 50 µm. T cells activated and expanded using AimGel at a 1:1 microparticle-to-cell ratio. Red signals indicate the AimGel microparticles. T cell AimGel clusters are visible on day 2 and enlarged by day 3. Red signals disappear as the microparticles begin degrading and dissociate from T cells on day 5. By day 7 the microparticles are degraded, releasing the T cells.

  • AimGel FAQs

  • Order Now

    Purchase through our distributors, or our webstore if no distributor is serving your region.

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