AimGel Human T Cell Activator
CD3/CD28/CD137 activation on cell-sized hydrogel microparticles
AimGel Human T Cell Activator
$150.00
ATC01-0050H | Research Use Only
AimGel Human T Cell Activator presents anti-CD3, anti-CD28 and anti-CD137 signals on soft hydrogel microparticles built to the size of a real cell (7-12 µm). The fluid lipid membrane lets those signals move freely across the surface, the way they do on a natural antigen-presenting cell — so activation is gentle and effective, whether you start from isolated T cells or straight from PBMC.
1 vial of AimGel Human T Cell Activator, 0.5 mL in PBS at 4 × 10⁷ microparticles/mLQuantityData
Proliferation and viability
When expanding T cells straight from PBMC, AimGel outperforms existing synthetic solutions such as magnetic beads and nanomatrix by 60%, yielding more cells in a shorter time. Viability of T cells activated via AimGel matches current synthetic solutions. n=4, PBMCs from healthy human donors.
More cells, same purity
When enriching directly from PBMC with AimGel, you get more cells, but matching the high T cell purity (90%) of common activation solutions.
Memory subtypes
T cell memory subtypes of PBMCs activated by AimGel and other popular T cell activation reagents. AimGel produces more even fractions of effector memory and central memory subtypes.
Activation markers
Expression of early-stage marker (CD69) and late-stage marker (CD25), indicating T cell activation. Unstimulated cells used as gating.
CAR-T function
In vitro CAR-T cell mediated killing assessed by fluorescent microscopy of GFP⁺ Raji cancer cell lines co-cultured with CAR-T cells.
Degradation timecourse
Bright-field micrographs showing activation and expansion of pan T cells at days 2, 3, 5 and 7. Scale bar = 50 µm. T cells activated with AimGel at a 1:1 microparticle-to-cell ratio. Red signals indicate the AimGel microparticles: clusters are visible on day 2 and enlarge by day 3, then the signal fades from day 5 as the microparticles degrade, leaving free T cells by day 7.
Shown with the hydrolysable formulation. Our activators
ship non-hydrolysable and are cleared with the included lysis buffer.
Degradation toxicity
AimGel microparticles, as well as the degradation products showed excellent biocompatibility and minimum cytotoxicity at working dosage, regardless of the degradation method: enzymatic (user-triggered via lysis buffer) or hydrolysis (available upon request). 10% DMSO used as control for killing the T cells.
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Hong Kong
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Hong Kong
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Mouse T Cell Activator
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Dog T Cell Activator
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Aim-Core
Blank artificial cells for custom applications. Dock your own biotinylated signals.
